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Over time, various stimuli, such as inflammatory signals (e.g., TGF- 106 ) or microenvironmental changes, 107 can trigger CSC reactivation, leading to tumor recurrence

Different types of formaldehyde-derived imines or iminium salts, which are generally unstable and have to be in situ generated from formaldehyde with aromatic amines 10,11 , -aminomethyl ethers 12,13,14,15,16,17 , N , O -acetals 18,19,20 , or 1,3,5-triaryl-1,3,5-triazines 21,22,23,24 , have been successfully applied

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Proteins were extracted using radioimmunoprecipitation assay lysis buffer (25 mM Tris-HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% sodium dodecyl sulfate) containing protease inhibitors

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3.2 Cellular therapy 3.2.1 Neuregulin1 With the development of stem cell research, the use of neural stem cells (NSCs) in the treatment of diseases is now of great interest