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Persistent bottlenecks include the increased complexity and risk of genome rearrangement caused by co-infection with auxiliary viruses, the lower virus particle yield in suspension cultures (3,000 to 8,000 particles per cell) compared to adherent systems (10,000 to 20,000 particles per cell), and the limited scalability and regulatory compliance of density gradient ultracentrifugation used for auxiliary virus isolation [282]

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In contrast, higher concentrations of microalgal extract had a suppressive impact on yield and growth