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These collinear relationships were distributed across 11 GST subfamilies, with GSTU, GSTF, and EF1G accounting for the largest numbers of members

For image analysis, regions of interest (ROIs) corresponding to imaged cells and Hoechst 33342-stained nuclei in each frame were automatically segmented by Cellpose algorithm using pretrained and custom-trained model nucleus

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This review synthesizes recent advances in understanding the molecular mechanisms underlying their interaction, focusing on how GLMDs drive ferroptosis by supplying lipid peroxidation substrates, inducing iron overload, and weakening antioxidant defenses