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Mechanistic insights into hyperuricemia-associated renal abnormalities with special emphasis on epithelial-to-mesenchymal transition: Pathologic implications and putative pharmacologic targets

To initially locate the most abundant phosphorylation sites within ArfGAP1, we conducted in vitro radioactive kinase assays using [ 33 P]--ATP together with immunopurified recombinant full-length FLAG-tagged human LRRK2 (WT, G2019S or D1994A) and a series of GST-tagged ArfGAP1 protein fragments (residues 1-136, 137-415, 137-251, 252-359 and 360-415) ( Fig

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Preparation The injection site, typically in the upper arm or thigh, will be cleaned to ensure sterility